Search results "dna-lego-kit-2"

  • Products
Column DNA Lego Kit, 200 izolations
Column DNA Lego Kit is a universal modular system (Lego-like) for pure DNA isolation from various sources. The method is based on capacity of silica surfaces to bind DNA in the presence of chaotropic agents (Proc. Natl. Acad. Sci USA, 76: 615-619, 1979).
PCR Genotyping Kit
PCR Genotyping Kit is designed for rapid extraction of DNA from various tissues and cells and for PCR genotyping of the extracted DNA. Kit includes DEP-25 DNA extraction Kit and Combi PPP Master Mix for Hot-Start PCR.
DEP-25 DNA Extraction Kit
DNA Extraction for PCR under 25 min (DEP-25) is a two-component reagent kit for extraction of genomic DNA of various origin.
DNA marker 200-1500
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500 (Fig. 1). These fragments were produced by the amplification of plasmid DNA of different length.
DNA marker 200-1500 coloured
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500 coloured (Fig. 1). These fragments were produced by the amplification of plasmid DNA of different length. DNA marker is supplemented with the dye and additives and therefore can be loaded directly into agarose gel.
DNA marker 200-1500+
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500+ (Fig. 1). These fragments were produced by amplification of plasmid DNA of different length.
DNA marker 200-1500+ col.
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500+ coloured (Fig. 1). These fragments were produced by amplification of plasmid DNA of different length.
RNase (DNase-free)
RNase (DNase-free) is isolated from bovine pancreas. This enzyme is suitable for isolation of plasmids using DNA Lego kit (Cat. No. D100)  
Q-Taq DNA polymeráza
Aptamer-Taq DNA polymerase
Aptamer-Taq DNA polymerase is a modification of Combi Taq DNA polymerase (Cat. No. C205-C207) in which DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody. When compared to antibody the aptamer has several advantages: (1) it is not irreversibly denatured at temperature upto 100oC, (2) has low molecular weight, is prepared synthetically, and has well defined composition, (3) is not contamined with components of mammalian cells, (4) is resistant to action of proteases and allows preparation of PCR mixes at room temperature, and (5) is less expensive.
Aptamer-Taq DNA polymerase, 5 U/µl
Aptamer-Taq DNA polymerase, 5 U/µl is a modification of Combi Taq DNA polymerase (Cat. No. C205-C207) in which DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody.
TP HS DNA-free 2x SYBR Master Mix
This Master Mix is dedicated for quantitative analysis of DNA samples using qPCR based on fluorescence dye SYBR Green I. The Master Mix is not contaminated with bacterial and fungal DNA and therefore is especially useful for quantification of bacterial and fungal DNA.
TP HS DNA-free 2x Master Mix
The Master Mix is not contaminated with bacterial and fungal DNA and therefore is especially useful for bacterial and fungal DNA genotyping.
Combi Taq DNA polymerase
Combi Taq DNA polymerase is a modification of Taq DNA polymerase Unis (Cat. No. T037-T039). The enzyme is supplemented with monoclonal antibody anti-Taq, which allows "hot-start" PCR. Furthermore, the polymerase is present at lower concentration (1 U/µl), which simplifies pipetting of the enzyme.
Coloured DNA marker 155-970
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is often in the range 150-1000 base pairs. This range is covered by DNA fragments, which are part of DNA marker 155-970 (Fig. 1). These fragments were produced by cutting a reference plasmid by restriction enzyme. This marker DNA marker is supplemented innert dye (Purple) and additives increasing density and therefore can be loaded directly into agarose gel. This marker is especially suitable for analysis of PCR products generated by Taq-Purple DNA polymerase (Cat. No. T 107-T109). 
DNA marker 155-970
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is often in the range 150-1000 base pairs. This range is covered by DNA fragments, which are part of DNA marker 155-970 (Fig. 1). These fragments were produced by cutting a reference plasmid by restriction enzyme.
LA DNA Polymerases Mix
LA DNA polymerases Mix is unique blend of two thermostable DNA polymerases. One of them is Taq DNA polymerase which is highly processive but lacks a 3’->5’ exonuclease proofreading activity, which accounts for relatively high error rate during DNA amplification. Second polymerase is less processive but possesses 3’->5’ exonuclease activity necessary for proofreading. The polymerases mix decreases error rate during synthesis of complementary DNA strands and preserves high speed of DNA synthesis.
Combi PPP Master Mix
Combi PPP Master Mix is dedicated for simplified routinely performed hot start PCR. It contains Taq DNA polymerase, deoxyribonucleotides, reaction buffer components, additives and monoclonal antibody anti-Taq. Samples for PCR are prepared by simple mixing Combi PPP Master Mix with target specific oligonucleotide primers, template DNA and water. Additives and the dye present in Combi PPP Master Mix allow direct loading of the PCR amplified samples into the gel without adding loading buffer. When compared to PPP Master Mix (Cat. No, P124 - P126), it contains a monoclonal antibody which binds to and inactivates enzymatic activity of Taq DNA polymerase. The master mix is not recommended for any applications in which fluorescence excitation is used, such as qPCR.
Taq DNA polymerase Unis
Taq DNA polymerase Unis is an alternative to Taq DNA polymerase (Cat. No. T032-T034). Difference between these two products is in difference in enzyme storage buffer which enhances stability and efficiency of the enzyme. Occasional amplification of nonspecific fragments can be prevented by enhanced dilution of the enzyme.
Taq DNA polymerase 1.1
This product is a modification of Taq DNA polymerase Unis (Cat. No. T 037-T 039). Difference between these two products rely in lower concentration of the polymerase (1 U/µl) , which makes easier pipetting of the enzyme. Furthermore, the enzyme is supplied with 10x concentrated PCR Blue buffer. This buffer contains ammonium sulfate, which contributes to broader tolerance of MgCl2 and lower production of nonspecific enzymes.
  • Product combinations
Column DNA Lego Kit, 200 izolations
Column DNA Lego Kit, 200 izolations - Package: 1 kit
Column DNA Lego Kit + plasmid supplement, 200 izolations
Column DNA Lego Kit + plasmid supplement, 200 izolations - Package: 1 kit.
PCR Genotyping Kit
PCR Genotyping Kit - Package: 1000 reakcí
PCR Genotyping Kit
PCR Genotyping Kit - Package: 40 assays
PCR Genotyping Kit
PCR Genotyping Kit - Package: 200 assays
DNA marker 200-1500+ col.
DNA marker 200-1500+ col. - Package: 50 µg/300 µl
DNA marker 200-1500+ col.
DNA marker 200-1500+ col. - Package: 5x 50 µg/300 µl
DNA marker 200-1500+ col.
DNA marker 200-1500+ col. - Package: 20x 50 µg/300 µl
DNA marker 200-1500
DNA marker 200-1500 - Package: 25 µg/250 µl
DNA marker 200-1500
DNA marker 200-1500 - Package: 5x 25 µg/250 µl
DNA marker 200-1500
DNA marker 200-1500 - Package: 20x 25 µg/250 µl
DNA marker 200-1500 coloured
DNA marker 200-1500 coloured - Package: 25 µg/300 µl
DNA marker 200-1500 coloured
DNA marker 200-1500 coloured - Package: 5x 25 µg/300 µl
DNA marker 200-1500 coloured
DNA marker 200-1500 coloured - Package: 20x 25 µg/300 µl
DNA marker 200-1500+
DNA marker 200-1500+ - Package: 50 µg/250 µl
DNA marker 200-1500+
DNA marker 200-1500+ - Package: 5x 50 µg/250 µl
DNA marker 200-1500+
DNA marker 200-1500+ - Package: 10x 50 µg/250 µl
Taq DNA polymerase
Taq DNA polymerase - Package: 500 U
Taq-Purple DNA polymerase + PCR dNTP mix
Taq-Purple DNA polymerase + PCR dNTP mix - Package: 500 U + 100 µl
Aptamer-Taq DNA polymerase, 5 U/µl
Aptamer-Taq DNA polymerase, 5 U/µl - Package: 5x 500 U