Other materials for PCR

It is specially adjusted ultrapure water DNase and RNase free. Usually, RNases are removed from H2O by the treatment with diethylpyrocarbonate (DEPC), which is then resolved by autoclaving. Degradation products of DEPC can negatively influence the course of RT-PCR and even PCR. In our product PCR Ultra H2O, RNases were removed by double affinite chromatography on special carrier. For this purpose PCR Ultra H2O does not contain any degradation products of DEPC and can be universally used for any work with RNA and in RT-PCR and PCR.

PCR H2O is ultrapure water (18 Mohm.cm, ultrafiltred), from which RNases were removed by treatment with  diethylpyrocarbonate and by autoclaving.

PCR dNTP mix is a water solution containing ultrapure mixture of each of the four dNTPs (dATP, dCTP, dGTP, dTTP), pH 7.5.

PCR Et-OH is 75% ethanol in ultrapure water (18 Mohm.cm, ultrafiltred), from which RNases were removed by the treatment with  diethylpyrocarbonate followed by autoclaving.

Amplification of DNA fragments by PCR often demands optimization of reaction conditions, which lead to the removal of undesirable unspecifities and to the increase of amount of PCR product. Optimization includes the concentration of Mg2+, concentration of polymerase, temperature of annealing of oligonucleotide primers and others. In some cases optimization can be reached by addition of additives (e.g. DMSO or formamide). Interesting effects have been obtained with tetramethyl ammonium (TMA) oxalate (Kovářová and Dráber, Nucleic Acid Res., 28: e70, 2000), which is available under the commercial name PCR Enhancer. This enhancer is can be added directly into PCR mixture.

96% ethyl alcohol in ultrapure water

Sodium acetate buffer solution, 3 M, pH 5.2, is of Molecular Biology Grade prepared.

It is a 200x concentrated blue dye that increases the visibility of PCR reaction mixes without affecting PCR efficiency and does not interfere with widely used fluorescent DNA dyes (e.g. SYBR Green) and probes used for qPCR.