RNase (DNase-free)

RNase (DNase-free) is isolated from bovine pancreas. This enzyme is suitable for isolation of plasmids using DNA Lego kit (Cat. No. D100)  

Cat.no. Name Package Price
D106 RNasa A, DNase-free (10 mg/ml) 0,5 ml € 33,18

Product description

RNase (DNase-free) is isolated from bovine pancreas. This enzyme is suitable for isolation of plasmids using DNA Lego kit (Cat. No. D100)  

Technical data

Enzyme Concentration

  • RNase (DNase-free) is supplied at a concentration 10 mg/ml; ~900 U/ml.

Components and Packaging

  • 1 test tube RNase (DNase-free), 5 000 U/500 µl.
  • 1 test tube with 5x concentrated react buffer, 1 ml.

Unit Definition

  • One unit corresponds to the amount of enzyme which hydrolyzes RNA at a rate constant  k = 1 at 25oC and pH 5.0 (M. Kunitz, J. Biol. Chem., 164: 563, 1946).              

Storage

  • At temperature -20oC ± 5°C. Material can be repeatedly defrosted.

Composition

  • Storage buffer: 20 mM Tris-HCl (pH 7.5 at 20oC), 30 mM NaCl, 50% (vol/vol) glycerol.

Quality control

  • Under conditions completely degrading RNA, the enzyme has no effect on DNA (as defined by electrophoresis in agarose gel).

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