PCR Sodium acetate buffer sol., 3 M, pH 5.2
Sodium acetate buffer solution, 3 M, pH 5.2, is of Molecular Biology Grade prepared.
|P053||PCR Sodium acetate buffer sol., 3 M, pH 5.2||100 ml||€ 24,78|
Sodium acetate buffer solution, 3 M, pH 5.2, is of Molecular Biology Grade prepared, filter-sterilized and autoclaved. It is suitable for ethanol or isopropanol precipitation of nucleic acids.
Components and packaging
- 3M Sodium acetate buffer solution, 3 M, pH 5.2 is supplied in plastic flasks containing 100 ml of the solution.
Storage and Stability
- At room temperature (15 -25°C). The product is stable until the expiration date printed on the flask label.
- 3 M Sodium acetate buffer solution, pH 5.2 ± 0.1 at 22oC. It is prepared with Sodium acetate of Molecular Biology Grade and PCR Ultra H2O (Cat. No. P040).
- Each batch of Sodium acetate buffer solution, 3 M, pH 5.2 is tested for nucleic acid precipitation and nuclease activity. Economy DNA marker (Cat. No. D071; 5 µl) is mixed with 0.2 ml 10 mM Tris buffer, pH 8.0 + 1 mM EDTA (Cat. No. P055), 1 µl Carrier-ACRYL (Cat. No. C081), 20 µl of Sodium acetate, 3 M, pH 5.2, and 0.6 ml of 96% Ethanol (Cat. No. P054). After 30 min incubation at -20 ± 5oC the mixture is centrifuged for 15 min at 12,000 x g, sediemnt is washed with 75% Ethanol, dissolved in PCR H2O and analyzed by electrophoresis in agarose gel with ethidium bromide. Economy DNA marker without precipitation is analyzed in paralel on the same gel. More than 90% of all components of the DNA marker is recovered in the precipitate.