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Materials for testing of RNA coronavirus, causing
COVID-19
This product is similar to qPCR 2x Blue Master Mix, but it is supplemented with ROX (6-carboxy-X-rhodamine) reference dye. ROX serves as an internal reference for normalization of the fluorescent signal when using real-time PCR instruments, which can detect ROX. ROX allows for correction of the well-to-well variation due to pipetting inaccuracies and fluorescence fluctuations. The presence of ROX does not interfere with qPCR amplification (G. Wang, E. Becker, C. Mesa, Can. J. Microbiol. 53, 391-397, 2007). ROX has different emission spectrum (the excitation/emission maxima are at 586 nm/605 nm, respectively) when compared to SYBR Green I and other probes used for qPCR analyses.
ROX Reference Dye ROX in qPCR mixes is used in two concentrations, depending on instrument used.
qPCR 2x Blue Master Mix + ROX 500 - Final concentration 500 nM when using Applied Biosystems 7000, 7300, 7700, 7900HT, StepOne, StepOnePlus and othr PCR machines.
qPCR 2x Blue Master Mix + ROX 50 - Final concentration 50 nM when using Applied Biosystems 7500, 7500Fast, Stratagene cyclers and other machines..
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