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RNase (DNase-free)

Description

RNase (DNase-free) is isolated from bovine pancreas. This enzyme is suitable for isolation of plasmids using DNA Lego kit (Cat. No. D100)  

Technical data

Enzyme Concentration

  • RNase (DNase-free) is supplied at a concentration 10 mg/ml; ~900 U/ml.

Components and Packaging

  • 1 test tube RNase (DNase-free), 5 000 U/500 µl.
  • 1 test tube with 5x concentrated react buffer, 1 ml.

Unit Definition

  • One unit corresponds to the amount of enzyme which hydrolyzes RNA at a rate constant  k = 1 at 25oC and pH 5.0 (M. Kunitz, J. Biol. Chem., 164: 563, 1946).              

Storage

  • At temperature -20oC ± 5°C. Material can be repeatedly defrosted.

Composition

  • Storage buffer: 20 mM Tris-HCl (pH 7.5 at 20oC), 30 mM NaCl, 50% (vol/vol) glycerol.

Quality control

  • Under conditions completely degrading RNA, the enzyme has no effect on DNA (as defined by electrophoresis in agarose gel).

Downloads

Cat. No. Product name and specification Amount Price
D106 RNase (DNase-free) 0.5 ml 29.17 EUR