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M-MLV Reverse Transcriptase

Description

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an enzyme for cDNA synthesis from single-stranded RNA in the presence of oligonucleotide primer.           

Technical data

Enzyme concentration

  • M-MLV RT is supplied at a concentration 200 U/μl.

Components and packaging

  • 1 tube with M-MLV RT; 2 000 U/10 µl.
  • 1 tube with 5x concentrated M-MLV RT react buffer, 200 µl.
  • 1 tube of 100 mM dithiothreitol (DTT), 100 µl.

Unit definition

  • One unit of M-MLV RT is defined as the amount of the enzyme which incorporates 1 nmol of dTTP into acid precipitable material during 10 min at 37oC;   poly(A)-oligo (dT)12-18 is used as a template.

Storage

  • At temperature -20oC  ± 5°C.

Purity

  • The enzyme is free of nucleases and is suitable for performing cDNA synthesis.

Composition

  • Storage buffer: 20 mM Tris-HCl (pH 7.5 at 22oC), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Nonidet P-40, 50% (vol/vol) glycerol.
  • 5x concentrated M-MLV RT buffer (1st-strand buffer): 250 mM Tris-HCl (pH 8.3 at 22oC), 375 mM KCl, 15 mM MgCl2, stabilizer.

Downloads

Cat. No. Product name and specification Amount Price
M023 M-MLV Reverse Transcriptase 2000 U 62.5 EUR
M024 M-MLV Reverse Transcriptase 5x 2000 U 250 EUR