News

Dear customers, We would like to inform you that in connection with increasing requirements for testing for the presence of coronavirus causing COVID-19, we have expanded our low-priced offer with the following high volume qPCR Master Mixes containing all PCR components except primers, probes, and template DNA/cDNA.

Dear customers,

we would like to inform you that in connection with increasing requirements for testing for the presence of coronavirus causing COVID-19, we have expanded our low-priced offer with the following high volume qPCR Master Mixes containing all PCR components except primers, probes, and template DNA/cDNA.

qPCR 2x Blue Master Mix

  • Cat. No. P523bl, 1600 reactions, 20ml, price: 275,- EUR

qPCR 2x Master Mix

  • Cat. No. P503bl, 1600 reactions, 20ml, price: 275,- EUR

LA Hot Start Plain Master Mix

  • Cat. No. L613bl, 1600 reactions, 20ml, price: 333,- EUR

These are the most commonly used 2x concentrated qPCR Master Mixes newly supplied in 50 ml tubes. Simply, add the selected primers and probes to the tubes, and after mixing, Master Mixes can be aliquoted in smaller volumes and frozen. Just before use, Master Mixes can be thawed, supplemented with reverse transcription (RT) products and water, and used for qPCR. 
 
We also remind you that we have silica particles “RNA/DNA bind” (Cat. No. D102) and various carriers for RNA isolation, including the most often used (carrier-ACRYL, Cat. No. C082).

Carrier-iRNA / RNA carrier, Carrier-ACRYL, Carrier-GLY 

  • For precipitation of small amounts of RNA or DNA. 
Table 1 - Comparison of various carriers for RNA/DNA precipitation.
Carrier Advantages Disadvantages

Carrier-iRNA

Polyinosinic acid

Chemically defined RNA, which is more suitable as carrier for cDNA synthesis and other RNA/DNA manipulations than widely used rRNAs or tRNAs.

Could inhibit reactions catalyzed by terminal transferase or polynucleotide kinase. Interferes with determination of RNA or DNA concentrations.

Carrier-ACRYL

Linear polyacrylamide

 

Inert neutral carrier, which does not inhibit DNA cloning, DNA-protein interactions, and enzyme reactions. Does not interfere with determination of RNA/DNA concentrations. Does not co-precipitate short oligonucleotides (≤ 20 pbs).

Does not co-precipitate short oligonucleotides (≤ 20 pbs).

Carrier-GLY

Polysaccharide (glycogen from oysters)

 

Purified glycogen does not inhibit DNA cloning and most enzyme reactions; does not interfere with determination of RNA/DNA concentrations. It is suitable as inert carrier for precipitation of shorter oligonucleotides (≥ 8 pbs). 

May inhibit some DNA-protein interactions and reverse transcription of long RNA templates.