Aptamer Hot Start 2x Master Mix
Aptamer Hot Start Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, DNA aptamer anti-Tag, dNTPs, dye, MgCl2, additives and stabilizers. Additives and the dye allow direct loading of the PCR-amplified samples into the gel without adding loading buffer. When compared to hot start master mix containing Taq polymerase-specific monoclonal antibody, aptamer-based hot start Master Mix has an advantage that
(1) it is more resistant to elevated temperatures,
(2) the inhibitory effect is reversible, and
(3) master mix is more chemically defined. The master mix is not recommended for applications in which fluorescent DNA probes are used for detection amplified DNA (qPCR). It is ideally suited for routine PCR amplification of DNA fragments up to 5 kb. It is compatible with template DNA extracted with DEP-25 DNA extraction kit.