High quality

Nejvyšší dosažitelná kvalita

We ensure careful final inspection independently on manufacturing. The products come with quality certificates, which are available in electronic form... 

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Certificate ISO PDF

We possess ISO 9001:2008 certificate: “Manufacturing high quality reagents...

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Firm’s testimony

  • Production of reagents of the highest quality
  • Demanding output control
  • Emphasis is put...

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News

Column DNA Lego Kit - modern system for pure DNA isolation

Aptamer Hot Start Master Mix - same price as normal PCR Master Mix

DEP-25 DNA Extraction Kit - DNA Extraction for PCR under 25 min

Master mixy 

Hot Start PCR Master Mixes

Aptamer Hot Start Master Mix - NEW 2014

  • Aptamer Hot Start Master Mix contains DNA aptamer anti-Taq which binds reversibly to Taq DNA polymerase, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. After PCR the aptamer regains its inhibitory activity.

Combi PPP Master Mix

  • A modified PPP master mix supplemented with anti-Taq monoclonal antibody, which inactivates enzymatic activity of Taq DNA polymerase. The enzyme is activated after denaturation of the antibody during denaturation phase of PCR cycle. This modification leads to "hot start PCR".

Plain Combi PP Master Mix

  • A modified Combi PPP Master Mix without a dye.

LA Hot Start Master Mix - New 2014

  • LA (Long and Accurate). The Mix contains unique blend of two thermostable DNA polymerases. One of them is Taq DNA polymerase, which is highly processive but lacks a 3’->5’ exonuclease proofreading activity, which accounts for relatively high error rate during DNA amplification. Second polymerase is less processive but possesses 3’->5’ exonuclease activity necessary for proofreading. The polymerases mix decreases error rate during synthesis of complementary DNA strands and preserves high speed of DNA synthesis. The repair capability allows amplification of complex genomic DNA fragments (up to 15 kbps in size) and less complex viral DNA (up to 40 kbps in size) and significantly decreases error rate when compared to Taq DNA polymerase.
  Hot Start

 

Inert Dye

Long
Acurate

MgCl2 incl.

PPP Master Mix

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+

-

+

PPP Master Mix bez MgCl2

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+

-

-

Plain PP Master Mix

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-

-

+

TP 2x Master Mix - - - +

Combi PPP Master Mix

+

+

-

+

Plain Combi PP Master Mix

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-

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+

LA Hot Start Master Mix + - + +

Aptamer Hot Start Master Mix

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