High quality

Nejvyšší dosažitelná kvalita

We ensure careful final inspection independently on manufacturing. The products come with quality certificates, which are available in electronic form... 

Read more

Certificate ISO PDF

We possess ISO 9001:2008 certificate: “Manufacturing high quality reagents...

Read more

Firm’s testimony

  • Production of reagents of the highest quality
  • Demanding output control
  • Emphasis is put...

Read more

News

Column DNA Lego Kit - modern system for pure DNA isolation

Aptamer Hot Start Master Mix - same price as normal PCR Master Mix

DEP-25 DNA Extraction Kit - DNA Extraction for PCR under 25 min

Aptamer-Taq DNA polymerase - NEW 2014

Description

Aptamer-Taq DNA polymerase is a modification of Combi Taq DNA polymerase (Cat. No. C205-C207) in which DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody. When compared to antibody the aptamer has several advantages:
(1) it is not irreversibly denatured at temperature upto 100oC,
(2) has low molecular weight, is prepared synthetically, and has well defined composition,
(3) is not contamined with components of mammalian cells,
(4) is resistant to action of proteases and allows preparation of PCR mixes at room temperature, and
(5) is less expensive.

Hot start

  • This products contains DNA aptamer anti-Taq, which binds specifically to Taq DNA polymerase and blocks its enzymatic activity until the first denaturation phase of PCR. At 94oC the antibody is irreversibly denatured and the activity of the polymerase is restored. This decreases formation of nonspecific DNA fragments during PCR.

Taq DNA polymerase

  • Taq DNA polymerase is a thermostable enzyme isolated from Thermus aquaticus. The enzyme catalyzes synthesis of complementary DNA strand in the 5’->3’ direction and also possesses a 5’->3’ exonuclease activity. During amplification of DNA fragments, Taq polymerase adds at 3’ end an adenosine overhang. This can be utilized for cloning of PCR-generated DNA fragments. Advantage of the enzyme is its high processivity (amplification of 1000 base pairs takes < 1 min). Disadvantage of the enzyme is that it lacks a 3’->5’ exonuclease proofreading activity and this accounts for high error rate [about 1 error to 105 - 106 base pairs (bps)]. The major usage of the enzyme is in diagnostic analysis  for amplification of DNA fragments up to 5000 bps.

Technical data

Components and packaging

  • Aptamer-Taq DNA polymerase is supplied at a concentration 1 U/µl. Basic packaging contains 1 tube with 500 U/500 µl (A025), 5 tubes with 500 U/500 µl (A026) or 10 tubes with 500 U/500 µl (A027).
  • Each tube of Aptamer-Taq DNA polymerase is accompanied by a tube with 10x concentrated react buffer with MgCl2 (1.5 ml). If different concentration of MgCl2 is required, a tube with 10x concentrated PCR Blue Buffer without MgCl2 (1.5 ml) and a tube with 25 mM MgCl2 (0.5 ml) should be ordered (Cat. No. T059).

Storage

  • At temperature -20oC ± 5°C. Material can be repeatedly defrosted.

Composition

  • Storage buffer: 20 mM Tris-HCl (pH 8.0 at 25oC), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Nonidet P-40, 0.5% Tween 20, 50% glycerol.
  • 10x reaction buffer: 750 mM Tris-HCl, pH 8.8 (at 25oC), 200 mM (NH4)2SO4, 0.1% Tween 20, 25 mM MgCl2.

Activity

  • One unit is defined as the amount of enzyme, which catalyzes incorporation of 10 nmol dNTPs within 30 min at 72oC into trichloracetic acid precipitable material. Reaction conditions are as follow: 10 mM Tris-HCl (pH 8.8 at 25oC), 50 mM KCl, 0.1% Triton X-100, 1.5 mM MgCl2, 200 µM dATP, dCTP, dGTP and [α-32P]dTTP, 50 µg/ml denatured cDNA, 0.5 µM primer and 0.2 – 0.5 U of enzyme in the volume of 50 µl.

Purity and quality control

  • Purity of Aptamer-Taq DNA polymerase is tested by SDS-PAGE. Enzyme migrates as a major band of 94 kDa. Material is nuclease free.
  • Each batch of Aptamer-Taq DNA polymerase is tested for its ability to amplify DNA fragment from mammalian genomic DNA, isolated with DEP-25 DNA extraction kit (Cat. No. D225-D227), by PCR. The results are verified by electrophoresis in agarose gel in the presence of ethidium bromide; only DNA band of the expected size is present.
 

Downloads

Cat. No. Product name and specification Amount Price
A025 Aptamer-Taq DNA polymerase 500 U 103.75 EUR
A026 Aptamer-Taq DNA polymerase 5x 500 U 415 EUR
A027 Aptamer-Taq DNA polymerase 10x 500 U 726.25 EUR