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10x concentrated PCR Blue Buffer

Description

PCR Blue Buffer contains ammonium sulfate instead of potassium chloride and thereby  exhibits higher tolerance towards changing concentrations of MgCl2. The reaction buffer can be used with the following Top-Bio polymerases: Taq DNA polymerase (Cat. No T032-T034), Taq DNA polymerase Unis (Cat. No. T037-T039), Taq-Purple DNA polymerase (Cat. No T107-T109), Taq DNA polymerase 1.1 (Cat. No T112-T114) and Combi Taq DNA polymerase (Cat. No C205-C207).

1x concentrated buffer contains 2.5 mM MgCl2, which is suitable for most PCRs. Alternatively, 10x concentrated buffer is provided without MgCl2 and 25 mM MgCl2 is provided separately. This allows rapid optimization of reaction conditions.

Technical data

Components and packaging

  • 1 test tube containing 10x concentrated PCR Blue Buffer (1.5 ml).
  • 10x concentrated PCR Blue buffer is either with 25 mM MgCl2 (Cat. No. T058) or without MgCl2 and 25 mM MgCl2 (0.5 ml) is provided in separate tube (Cat. No. T059).

Storage

  • At temperature -20oC ± 5°C. Material can be repeatedly defrosted.

Composition

  • 10x concentrated PCR Blue buffer (T058): 750 mM Tris-HCl (pH 8.8 at 25oC), 200 mM (NH4)2SO4, 1% Tween 25 mM MgCl2.
  • 10x concentrated PCR Blue buffer without MgCl2(T059): 750 mM Tris-HCl (pH 8.8 at 25oC), 200 mM (NH4)2SO4, 1% Tween 25 mM. 25 mM MgCl2 is in separate tube.

Purity and quality control

  • Material is nuclease free.
  • Each batch PCR Blue buffer is tested for its ability to amplify DNA fragment from mammalian genomic DNA by PCR. The results are evaluated by electrophoresis in agarose gel in the presence of ethidium bromide; only the expected DNA band is present.

Downloads

Cat. No. Product name and specification Amount Price
T058 10x conc. PCR Blue Buffer 1.5 ml 2.08 EUR
T059 10x conc. PCR Blue Buffer without MgCl2+MgCl2 1.5 ml + 0.5 ml 2.5 EUR