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Materials for testing of RNA coronavirus, causing
COVID-19
PCR Blue Buffer contains ammonium sulfate instead of potassium chloride and thereby exhibits higher tolerance towards changing concentrations of MgCl2. The reaction buffer can be used with the following Top-Bio polymerases: Taq DNA polymerase (Cat. No T032-T034), Taq DNA polymerase Unis (Cat. No. T037-T039), Taq-Purple DNA polymerase (Cat. No T107-T109), Taq DNA polymerase 1.1 (Cat. No T112-T114) and Combi Taq DNA polymerase (Cat. No C205-C207).
1x concentrated buffer contains 2.5 mM MgCl2, which is suitable for most PCRs. Alternatively, 10x concentrated buffer is provided without MgCl2 and 25 mM MgCl2 is provided separately. This allows rapid optimization of reaction conditions.
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