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Materials for testing of RNA coronavirus, causing
COVID-19
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500 coloured (Fig. 1). These fragments were produced by the amplification of plasmid DNA of different length. DNA marker is supplemented with the dye and additives and therefore can be loaded directly into agarose gel.
Fig. 1. Electrophoretic separation of components of DNA marker 200-1500 coloured.
10 µl of DNA marker coloured was directly loaded into wells of 1.2% agarose gel, which contained ethidium bromide (1µg/ml) and 1x TBE buffer. The numbers on the left represent the number of base pairs in the corresponding DNA fragments.
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