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Materials for testing of RNA coronavirus, causing
COVID-19
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on the comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is usually in the range 200-1500 base pairs. This range is covered by DNA fragments, which are part of DNA marker 200-1500+ (Fig. 1). These fragments were produced by amplification of plasmid DNA of different length.
Figure. 1. Electrophoretic separation of DNA marker 200-1500+.
10 µl of DNA marker was mixed with 3 µl of PCR loading buffer. Fragments were separated in 1.2% agarose gel, which contained ethidium bromid (1 µg/ml) and 1x TBE buffer. The numbers represent the number of base pairs in corresponding DNA fragments.
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