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Materials for testing of RNA coronavirus, causing
COVID-19
Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is often in the range 150-1000 base pairs. This range is covered by DNA fragments, which are part of DNA marker 155-970 (Fig. 1). These fragments were produced by cutting a reference plasmid by restriction enzyme. This marker DNA marker is supplemented innert dye (Purple) and additives increasing density and therefore can be loaded directly into agarose gel. This marker is especially suitable for analysis of PCR products generated by Taq-Purple DNA polymerase (Cat. No. T 107-T109).
Fig. 1. Electrophoretic separation of components of DNA marker 155-970 coloured.
10 µl of DNA marker coloured was directly loaded into wells of 1.2% agarose gel, which contained ethidium bromide (1µg/ml) and 1x TBE buffer. DNA fragments were separated by electrophoresis. The numbers represent the number of base pairs in the corresponding DNA fragments.
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