• DNA polymerases and buffers
  • Taq DNA polymerase
  • Taq DNA polymerase Unis
  • Taq-Purple DNA polymerase
  • Taq DNA polymerase 1.1
  • LA DNA Polymerases Mix
  • 10x concentrated PCR Blue Buffer
  • Survey of the products
• Hot Start DNA polymerases
  • Combi Taq DNA polymerase
  • Aptamer-Taq DNA polymerase
  • Survey of the products
• PCR Master Mixes
  • PPP Master Mix
  • PPP Master Mix without MgCl2
  • Plain PP Master Mix
  • TP 2x Master Mix
  • Survey of the products
• Hot Start PCR Master Mixes
  • Aptamer Hot Start Master Mix
  • Combi PPP Master Mix
  • Plain Combi PP Master Mix
  • LA Hot Start Master Mix
  • Survey of the products
• Real-time PCR (qPCR) Master Mixes
  • TP SYBR 2x Master Mix
  • qPCR 2x SYBR Master Mix
  • qPCR 2x SYTO-9 Master Mix
  • qPCR 2x Master Mix
  • qPCR 2x Blue Master Mix
  • LA Hot Start Plain Master Mix
  • ROX Reference Dye
  • qPCR 2x Blue Master Mix + ROX
  • Survey of the products
• Other enzymes
  • M-MLV Reverse Transcriptase
  • DNase I (RNase-free)
  • RNase (DNase-free)
  • Survey of the products
• DNA and RNA isolation
  • Column DNA Lego Kit
  • DEP-25 DNA Extraction Kit
  • PCR Genotyping Kit
  • RNA Blue
  • DNA Lego kit
  • Carrier-iRNA / RNA carrier
  • Carrier-ACRYL
  • Carrier-GLY
  • Survey of the products
• DNA markers
  • DNA marker 200-1500
  • DNA marker 200-1500 coloured
  • DNA marker 200-1500+ (200-700 po 50pb)
  • DNA marker 200-1500+ coloured (200-700 po 50pb)
  • DNA marker 155-970
  • DNA marker 155-970 coloured
  • Survey of the products
• Other materials for PCR
  • PCR dNTP mix
  • PCR enhancer
  • PCR H2O
  • PCR Ultra H2O
  • PCR Oil
  • PCR Et-OH
  • Survey of the products
• Materials for electrophoresis
  • PCR agarose
  • PCR ethidium bromide
  • PCR loading buffer
  • PCR loading buffer Red load
  • PCR loading buffer Yellow load
  • PCR loading buffer Edge
  • Survey of the products

DNA marker 155-970 coloured

Description

Estimation of the size of DNA fragments generated in PCR (PCR fragments) or by DNA cutting by restriction enzymes (restriction fragments) is usually based on comparison of these fragments with DNA fragments of known size (DNA markers). Size of PCR fragments is often in the range 150-1000 base pairs. This range is covered by DNA fragments, which are part of DNA marker 155-970 (Fig. 1). These fragments were produced by cutting a reference plasmid by restriction enzyme. This marker DNA marker is supplemented innert dye (Purple) and additives increasing density and therefore can be loaded directly into agarose gel. This marker is especially suitable for analysis of PCR products generated by Taq-Purple DNA polymerase (Cat. No. T 107-T109). 

Technical data

Concentration

• 1 µg DNA/12 µl buffer (25 mM potassium acetate, 10mM Tris acetate and 25 mM EDTA).

Packaging

• 1 tube containing 25 µg of restriction DNA fragments in  300 µl of buffer. This amount enables 30 analyses (each 10 µl).

Storage

• Store at temperature -20 ± 4^oC. Material can be repeatedly defrosted.

Quality control

• The presence of corresponding fragments is controlled by electrophoresis in agarose gel supplemented with ethidium bromide (1 µg/ml). When observed under UV light, 9 DNA fragments are observed (155, 194, 239, 305, 447,544,595,750, and 970 bp) as shown in Fig. 1.

Fig. 1. Electrophoretic separation of components of DNA marker 155-970 coloured.
10 µl of DNA marker coloured was directly loaded into wells of 1.2% agarose gel, which contained ethidium bromide (1µg/ml) and 1x TBE buffer. DNA fragments were separated by electrophoresis. The numbers represent the number of base pairs in the corresponding DNA fragments.

Downloads

Product Information