High quality

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We ensure careful final inspection independently on manufacturing. The products come with quality certificates, which are available in electronic form... 

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Certificate ISO PDF

We possess ISO 9001:2008 certificate: “Manufacturing high quality reagents...

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Firm’s testimony

  • Production of reagents of the highest quality
  • Demanding output control
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RNA Blue

REAGENT FOR RAPID ISOLATION OF PURE AND INTACT RNA

Description

RNA Blue is a ready to use reagent for progressive method of isolation of total RNA or for the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. Basis of the method has been described by Chomczynski and Sacchi (Anal. Biochem. 162, 156-159, 1987). This highly reliable technique performs well with small and large quantities of tissues or cultured cells, and allows simultaneous processing of a large number of samples.

RNA Blue reagent contains phenol and guanidine thiocyanate in a mono-phase solution. A biological sample is homogenized or lysed in RNA Blue reagent and the homogenate is separated into the aqueous and organic phases by chloroform addition and centrifugation. RNA remains exclusively in the aqueous phase, DNA in the interphase, and proteins remain in the organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol, washed with ethanol and solubilized. DNA and proteins are sequentially precipitated from the interphase and organic phase with ethanol and isopropanol, washed with ethanol and solubilized.

Technical data

Components an packing

  • Bottles containing 25, 50 or 100 ml of RNA Blue.
  • Detailed instructions for isolation of RNA, DNA and proteins from the same sample.

Storage

  • Store at temperature 4 ± 3oC.

Quality control

  • Each batch of RNA Blue is controlled for performance in RNA isolation. When RNA is isolated according the recommended protocol, RNA is intact as can be documented by electrophoretic separation on formaldehyde gel and staining with ethidium bromide.

Downloads

Cat. No. Product name and specification Amount Price
R011 RNA Blue 25 ml 41.25 EUR
R012 RNA Blue 50 ml 62.08 EUR
R013 RNA Blue 100 ml 116.67 EUR