• DNA polymerases and buffers
  • Taq DNA polymerase
  • Taq DNA polymerase Unis
  • Taq-Purple DNA polymerase
  • Taq DNA polymerase 1.1
  • LA DNA Polymerases Mix
  • 10x concentrated PCR Blue Buffer
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• Hot Start DNA polymerases
  • Combi Taq DNA polymerase
  • Aptamer-Taq DNA polymerase
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• PCR Master Mixes
  • PPP Master Mix
  • PPP Master Mix without MgCl2
  • Plain PP Master Mix
  • TP 2x Master Mix
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• Hot Start PCR Master Mixes
  • Aptamer Hot Start Master Mix
  • Combi PPP Master Mix
  • Plain Combi PP Master Mix
  • LA Hot Start Master Mix
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• Real-time PCR (qPCR) Master Mixes
  • TP SYBR 2x Master Mix
  • qPCR 2x SYBR Master Mix
  • qPCR 2x SYTO-9 Master Mix
  • qPCR 2x Master Mix
  • qPCR 2x Blue Master Mix
  • LA Hot Start Plain Master Mix
  • ROX Reference Dye
  • qPCR 2x Blue Master Mix + ROX
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• Other enzymes
  • M-MLV Reverse Transcriptase
  • DNase I (RNase-free)
  • RNase (DNase-free)
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• DNA and RNA isolation
  • Column DNA Lego Kit
  • DEP-25 DNA Extraction Kit
  • PCR Genotyping Kit
  • RNA Blue
  • DNA Lego kit
  • Carrier-iRNA / RNA carrier
  • Carrier-ACRYL
  • Carrier-GLY
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• DNA markers
  • DNA marker 200-1500
  • DNA marker 200-1500 coloured
  • DNA marker 200-1500+ (200-700 po 50pb)
  • DNA marker 200-1500+ coloured (200-700 po 50pb)
  • DNA marker 155-970
  • DNA marker 155-970 coloured
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• Other materials for PCR
  • PCR dNTP mix
  • PCR enhancer
  • PCR H2O
  • PCR Ultra H2O
  • PCR Oil
  • PCR Et-OH
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• Materials for electrophoresis
  • PCR agarose
  • PCR ethidium bromide
  • PCR loading buffer
  • PCR loading buffer Red load
  • PCR loading buffer Yellow load
  • PCR loading buffer Edge
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DNA Lego kit

A KIT FOR RAPID ISOLATION OF DNA

Description

DNA Lego kit is a modern modular system (Lego-like) for isolation of DNA from various sources. The method is based on capacity of silica microparticles to bind DNA in the presence of chaotropic agents (Proc. Natl. Acad. Sci USA, 76: 615-619, 1979). When compared to some other methods of DNA isolation (Ausubel, F.M. et al., eds. Current Protocols in Molecular Biology, Vol. 3, Green Publishing Assoc. and Wiley-Interscience, New York, 1995), DNA isolation with DNA Lego kit allows to isolate DNA faster (does not require ethanol precipitation and drying), safer (does not require phenol extraction) and purer (no contamination with proteins RNA and its cleavage products). DNA isolated using DNA Lego kit can be used for variety of molecular-biology techniques such as sequencing, PCR, restriction analysis, mutagenesis in vitro, transcription in vitro transformation and transfection. Components of the kit can be used for DNA concentration and isolation of genomic DNA fragments from cells, crude blood samples, gels or solutions.   

Technical data

Unit Definition

• DNA Lego kit allows 250 isolations  (e.g. plasmids from bacterial cultures, 1.5 ml each). 

Storage

• At room temperature.

Kit Components and packaging

• DNA bind (silica microparticles), 25 ml
• DNA binding buffer, 150 ml
• Washing buffer, 500 ml
• Minicolumns/barrels, 25/250 pcs
• Instructions for DNA isolation, and preparation of solutions used for plasmid DNA isolation

User supplied materials

For isolation of DNA with DNA Lego kit, only commonly used laboratory equipment is used. Some solutions required for DNA isolation can be easily prepared in the laboratory according to the recipes at the end of this protocol. For rapid isolation of DNA we recommend to use "Vac-Man laboratory vacuum manifold" from Promega Corporation; this equipment allows simultaneous processing of 20 samples and efficiently replaces repeated centrifugation steps or using syringes.

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Product Information