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Column DNA Lego Kit - modern system for pure DNA isolation
Aptamer Hot Start Master Mix - same price as normal PCR Master Mix
DEP-25 DNA Extraction Kit - DNA Extraction for PCR under 25 min
DNA Lego kit
A KIT FOR RAPID ISOLATION OF DNA
Description
DNA Lego kit is a modern modular system (Lego-like) for isolation of DNA from various sources. The method is based on capacity of silica microparticles to bind DNA in the presence of chaotropic agents (Proc. Natl. Acad. Sci USA, 76: 615-619, 1979). When compared to some other methods of DNA isolation (Ausubel, F.M. et al., eds. Current Protocols in Molecular Biology, Vol. 3, Green Publishing Assoc. and Wiley-Interscience, New York, 1995), DNA isolation with DNA Lego kit allows to isolate DNA faster (does not require ethanol precipitation and drying), safer (does not require phenol extraction) and purer (no contamination with proteins RNA and its cleavage products). DNA isolated using DNA Lego kit can be used for variety of molecular-biology techniques such as sequencing, PCR, restriction analysis, mutagenesis in vitro, transcription in vitro transformation and transfection. Components of the kit can be used for DNA concentration and isolation of genomic DNA fragments from cells, crude blood samples, gels or solutions.
Technical data
Unit Definition
- DNA Lego kit allows 250 isolations (e.g. plasmids from bacterial cultures, 1.5 ml each).
Storage
- At room temperature.
Kit Components and packaging
- DNA bind (silica microparticles), 25 ml
- DNA binding buffer, 150 ml
- Washing buffer, 500 ml
- Minicolumns/barrels, 25/250 pcs
- Instructions for DNA isolation, and preparation of solutions used for plasmid DNA isolation
User supplied materials
For isolation of DNA with DNA Lego kit, only commonly used laboratory equipment is used. Some solutions required for DNA isolation can be easily prepared in the laboratory according to the recipes at the end of this protocol. For rapid isolation of DNA we recommend to use "Vac-Man laboratory vacuum manifold" from Promega Corporation; this equipment allows simultaneous processing of 20 samples and efficiently replaces repeated centrifugation steps or using syringes.
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Product Information
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