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Materials for testing of RNA coronavirus, causing
COVID-19
DNA Lego kit is a modern modular system (Lego-like) for isolation of DNA from various sources. The method is based on capacity of silica microparticles to bind DNA in the presence of chaotropic agents (Proc. Natl. Acad. Sci USA, 76: 615-619, 1979). When compared to some other methods of DNA isolation (Ausubel, F.M. et al., eds. Current Protocols in Molecular Biology, Vol. 3, Green Publishing Assoc. and Wiley-Interscience, New York, 1995), DNA isolation with DNA Lego kit allows to isolate DNA faster (does not require ethanol precipitation and drying), safer (does not require phenol extraction) and purer (no contamination with proteins RNA and its cleavage products). DNA isolated using DNA Lego kit can be used for variety of molecular-biology techniques such as sequencing, PCR, restriction analysis, mutagenesis in vitro, transcription in vitro transformation and transfection. Components of the kit can be used for DNA concentration and isolation of genomic DNA fragments from cells, crude blood samples, gels or solutions.
For isolation of DNA with DNA Lego kit, only commonly used laboratory equipment is used. Some solutions required for DNA isolation can be easily prepared in the laboratory according to the recipes at the end of this protocol. For rapid isolation of DNA we recommend to use "Vac-Man laboratory vacuum manifold" from Promega Corporation; this equipment allows simultaneous processing of 20 samples and efficiently replaces repeated centrifugation steps or using syringes.
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