High quality

Nejvyšší dosažitelná kvalita

We ensure careful final inspection independently on manufacturing. The products come with quality certificates, which are available in electronic form... 

Read more

Certificate ISO PDF

We possess ISO 9001:2008 certificate: “Manufacturing high quality reagents...

Read more

Firm’s testimony

  • Production of reagents of the highest quality
  • Demanding output control
  • Emphasis is put...

Read more

News

Materials for testing of RNA coronavirus, causing
COVID-19

Carrier-iRNA / RNA carrier

For precipitation of small amounts of RNA or DNA (previously called RNA carrier R057)

Description

Carrier-iRNA is polyinosinic acid of Molecular Biology Grade. It is an efficient inert carrier for ethanol precipitation of picograms and higher quantities of RNA or DNA. Carrier-iRNA offers several advantages over other carriers, such as tRNA, yeast RNA, or sonicated DNA, for recovering nucleic acids prior to downstream applications. Carrier-iRNA is synthetic polymer, which is not source of biological contamination in the samples. Nucleic acids recovered after precipitation in the presence of Carrier-iRNA are immediately suitable for downstream applications such as PCR and  RT-PCR. 

Technical data

Components and packaging

  • Carrier-iRNA is supplied in deionized, ultrapure, and sterile water (18 Mohm.cm) at a concentration of ~10 mg/ml.
  • Basic packaging contains 0,5 ml of Carrier-iRNA in 2 ml plastic vials with screw cap.
  • Carrier-iRNA is a part of the set for RNA or DNA precipitations, containing besides Carrier-iRNA also Carrier-ACRYL and Carrier-GLY. Comparison of various carriers for RNA or DNA precipitation and key references are shown in Table 1

Storage and Stability

  • Store at temperature -20°C ± 5°C. Carrier-iRNA is stable until the expiration date printed on the tube label. To reduce the viscosity after freezing, we recommend heating the Carrier-iRNA tube to 37°C for 15 min.

Quality control

Each batch of Carrier-iRNA is analyzed in several assays. For the assays, DNA or RNA is examined in the Carrier Assay Buffer (CAB): 10 mM Tris-HCl, 2 mM MgCl2, 1 mM dithiothreitol, pH 7.5 at 37°C. 

  • Nucleic acid precipitation assay. Economy DNA marker (Cat. No. D071; 2.5 µl) is mixed with 0.2 ml 10 mM Tris buffer, pH 8.0 + 1 mM EDTA, 1 µl Carrier-iRNA, 20 µl of 3 M sodium acetate, pH 5.2, and 0.6 ml of 96% Ethanol. After 30 minutes at 2 - 8°C the mixture is centrifuged for 10 min at 12,000 x g, analyzed by electrophoresis in agarose gel with ethidium bromide and observed under UV light. More than 90% of all components of the DNA marker is recovered in the precipitate.
  • Nick activity assay. Plasmid pUC19 (1 µg) in 0.2 ml CAB is incubated with Carrier-iRNA (50 µg) for 1 hours at 37°C, followed by electrophoresis in agarose gel with ethidium bromide. No nicking activity is observed. 
  • Ribonuclease assay. RNA (1 µg) in 50 µl CAB with Carrier-iRNA (50 µg) is incubated for 1 hours at 37°C, followed by electrophoresis in agarose gel with ethidium bromide. No changes in properties of RNA are observed under UV light.
Table 1 - Comparison of various carriers for RNA/DNA precipitation.
Carrier Advantages Disadvantages

Carrier-iRNA

Polyinosinic acid

Chemically defined RNA, which is more suitable as carrier for cDNA synthesis and other RNA/DNA manipulations than widely used rRNAs or tRNAs.

Could inhibit reactions catalyzed by terminal transferase or polynucleotide kinase. Interferes with determination of RNA or DNA concentrations.

Carrier-ACRYL

Linear polyacrylamide

 

Inert neutral carrier, which does not inhibit DNA cloning, DNA-protein interactions, and enzyme reactions. Does not interfere with determination of RNA/DNA concentrations. Does not co-precipitate short oligonucleotides (≤ 20 pbs).

Does not co-precipitate short oligonucleotides (≤ 20 pbs).

Carrier-GLY

Polysaccharide (glycogen from oysters)

 

Purified glycogen does not inhibit DNA cloning and most enzyme reactions; does not interfere with determination of RNA/DNA concentrations. It is suitable as inert carrier for precipitation of shorter oligonucleotides (≥ 8 pbs). 

May inhibit some DNA-protein interactions and reverse transcription of long RNA templates.
 
 

Downloads

Cat. No. Product name and specification Amount Price
C078 Carrier-iRNA, 10 mg/ml 0,5 ml 70.83 EUR
C079 Carrier-iRNA, 10 mg/ml 5x 0,5 ml 249.58 EUR