High quality

Nejvyšší dosažitelná kvalita

We ensure careful final inspection independently on manufacturing. The products come with quality certificates, which are available in electronic form...

Certificate ISO

We possess ISO 9001:2008 certificate: “Manufacturing high quality reagents...

Production of reagents of the highest quality
Demanding output control
Emphasis is put...

News

Materials for testing of RNA coronavirus, causing
COVID-19

Carrier-ACRYL

For precipitation of small amounts of RNA or DNA

Description

Carrier-ACRYL is linear polyacrylamide (LPA) of Molecular Biology Grade. It is an efficient inert carrier for ethanol precipitation of picograms and higher quantities of RNA or DNA. Carrier-ACRYL offers several advantages over other carriers, such as tRNA, yeast RNA, or sonicated DNA, for recovering nucleic acids prior to downstream applications. Carrier-ACRYL is synthetic polymer, which is not source of biological contamination in the samples. Presence of Carrier-ACRYL during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides. This feature makes Carrier-ACRYL useful for separating reactions products from unincorporated nucleotides and oligonucleotide primers. Nucleic acids recovered after precipitation in the presence of Carrier-ACRYL are immediately suitable for downstream applications such as PCR, RT-PCR, restriction digestion, ligation, and sequencing.

Technical data

Components and packaging

Carrier-ACRYL is supplied in deionized, ultrapure, and sterile water (18 Mohm.cm) at a concentration of ~25 mg/ml.
Basic packaging contains 1 ml of Carrier-ACRYL in 2 ml plastic vials with screw cap.
Carrier-ACRYL is a part of the set for RNA or DNA precipitations, containing besides Carrier-ACRYL also Carrier-iRNA and Carrier-GLY, 1 ml each. Comparison of various carriers for RNA or DNA precipitation and key references are shown in Table 1.

Storage and Stability

For short terms (months) at temperature 4°C ± 3°C.
For long terms at temperature -20°C ± 5°C.

Quality control

Each batch of Carrier-ACRYL is analyzed in several assays. For the assays, DNA or RNA is examined in the Carrier Assay Buffer (CAB): 10 mM Tris-HCl, 2 mM MgCl₂, 1 mM dithiothreitol, pH 7.5 at 37^oC.

Nucleic acid precipitation assay. Economy DNA marker (Cat. No. D071; 2.5 µl) is mixed with 0.2 ml 10 mM Tris buffer, pH 8.0 + 1 mM EDTA, 1 µl Carrier-ACRYL, 20 µl of 3 M sodium acetate, pH 5.2, and 0.6 ml of 96% Ethanol. After 30 minutes at 2 - 8^oC the mixture is centrifuged for 10 min at 12,000 x g, analyzed by electrophoresis in agarose gel with ethidium bromide and observed under UV light. More than 90% of all components of the DNA marker is recovered in the precipitate.
Nick activity assay. Plasmid pUC19 (1 µg) in 0.2 ml CAB is incubated with Carrier-ACRYL (50 µg) for 1 hours at 37^oC, followed by electrophoresis in agarose gel with ethidium bromide. No nicking activity is observed.
Ribonuclease assay. RNA (1 µg) in 50 µl CAB with Carrier-ACRYL (50 µg) is incubated for 1 hours at 37^oC, followed by electrophoresis in agarose gel with ethidium bromide. No changes in properties of RNA are observed under UV light.
Absence of nucleic acids. Carrier-ACRYL (200 µg) is loaded on agarose gel with ethidium bromide. After electrophoresis, no bands are observed under UV light.

Table 1 - Comparison of various carriers for RNA/DNA precipitation.
Carrier	Advantages	Disadvantages
Carrier-iRNA Polyinosinic acid	Chemically defined RNA, which is more suitable as carrier for cDNA synthesis and other RNA/DNA manipulations than widely used rRNAs or tRNAs.	Could inhibit reactions catalyzed by terminal transferase or polynucleotide kinase. Interferes with determination of RNA or DNA concentrations.
Carrier-ACRYL Linear polyacrylamide	Inert neutral carrier, which does not inhibit DNA cloning, DNA-protein interactions, and enzyme reactions. Does not interfere with determination of RNA/DNA concentrations. Does not co-precipitate short oligonucleotides (≤ 20 pbs).	Does not co-precipitate short oligonucleotides (≤ 20 pbs).
Carrier-GLY Polysaccharide (glycogen from oysters)	Purified glycogen does not inhibit DNA cloning and most enzyme reactions; does not interfere with determination of RNA/DNA concentrations. It is suitable as inert carrier for precipitation of shorter oligonucleotides (≥ 8 pbs).	May inhibit some DNA-protein interactions and reverse transcription of long RNA templates.