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Materials for testing of RNA coronavirus, causing
COVID-19
Carrier-ACRYL is linear polyacrylamide (LPA) of Molecular Biology Grade. It is an efficient inert carrier for ethanol precipitation of picograms and higher quantities of RNA or DNA. Carrier-ACRYL offers several advantages over other carriers, such as tRNA, yeast RNA, or sonicated DNA, for recovering nucleic acids prior to downstream applications. Carrier-ACRYL is synthetic polymer, which is not source of biological contamination in the samples. Presence of Carrier-ACRYL during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides. This feature makes Carrier-ACRYL useful for separating reactions products from unincorporated nucleotides and oligonucleotide primers. Nucleic acids recovered after precipitation in the presence of Carrier-ACRYL are immediately suitable for downstream applications such as PCR, RT-PCR, restriction digestion, ligation, and sequencing.
For short terms (months) at temperature 4°C ± 3°C.
For long terms at temperature -20°C ± 5°C.
Each batch of Carrier-ACRYL is analyzed in several assays. For the assays, DNA or RNA is examined in the Carrier Assay Buffer (CAB): 10 mM Tris-HCl, 2 mM MgCl2, 1 mM dithiothreitol, pH 7.5 at 37oC.
Table 1 - Comparison of various carriers for RNA/DNA precipitation. | ||
Carrier | Advantages | Disadvantages |
Polyinosinic acid |
Chemically defined RNA, which is more suitable as carrier for cDNA synthesis and other RNA/DNA manipulations than widely used rRNAs or tRNAs. |
Could inhibit reactions catalyzed by terminal transferase or polynucleotide kinase. Interferes with determination of RNA or DNA concentrations. |
Linear polyacrylamide |
Inert neutral carrier, which does not inhibit DNA cloning, DNA-protein interactions, and enzyme reactions. Does not interfere with determination of RNA/DNA concentrations. Does not co-precipitate short oligonucleotides (≤ 20 pbs). |
Does not co-precipitate short oligonucleotides (≤ 20 pbs). |
Polysaccharide (glycogen from oysters)
|
Purified glycogen does not inhibit DNA cloning and most enzyme reactions; does not interfere with determination of RNA/DNA concentrations. It is suitable as inert carrier for precipitation of shorter oligonucleotides (≥ 8 pbs). |
May inhibit some DNA-protein interactions and reverse transcription of long RNA templates. |
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